Skeletal Muscle Mitochondrial Subpopulation Response in a Type 2 Diabetic Mouse Model
Bibliographic record
Abstract
Dysfunctional mitochondria are central to the pathogenesis of type 2 diabetes mellitus. In skeletal muscle, two spatially distinct mitochondrial subpopulations exist. The interfibrillar mitochondria (IFM) which are situated between myofibrils and the subsarcolemmal mitochondria (SSM) which reside directly below the sarcolemma. The objective of this study was to determine how type 2 diabetes mellitus differentially affects distinct mitochondrial subpopulations in skeletal muscle. Db/db mice and littermate controls were sacrificed at 20 weeks of age and mitochondrial subpopulations were isolated from the gastrocnemius muscle. State 3 respiration in db/db mice was significantly lower in both IFM and SSM populations compared to respective controls leading to a decrease in RCR ratios (P<0.05). Electron transport chain complex I and IV activities were significantly decreased in the diabetic IFM with no change in the SSM (P<0.05 for both). Superoxide production was increased in the diabetic IFM and SSM, although to a greater extent in the IFM. These findings indicate that both mitochondrial subpopulations in mouse gastrocnemius muscle are affected during type 2 diabetes mellitus, however, the IFM exhibit a greater dysfunctional profile.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".