The five transmembrane vasoactive intestinal peptide receptor 1 isoform is expressed in human blood CD14+ monocyte cells
Bibliographic record
Abstract
Vasoactive intestinal peptide receptor 1 (VPAC1) is a 7 transmembrane (TM) G protein coupled receptor that couples to Gα s and increases intracellular cAMP levels in response to external stimuli. Recently, a novel VPAC1 isoform was identified in human peripheral blood mononuclear cells (PBMC) that lacks exons 10‐12 to form a 5TM receptor that lacks Gα s coupling, but elicits significant tyrosine phosphorylation when treated with exogenous ligand in ectopically overexpressed CHO cells. VPAC1 is a deactivating factor for peripheral blood monocytes by inhibiting the production of many proinflammatory cytokines, but fails to increase intracellular cAMP levels. We hypothesize dual expression of 5 and 7TM VPAC1 may act antagonistically with regard to intracellular cAMP elevation. Uncovering the expression profile of the 5TM VPAC1 splice variant is a necessary first step at understanding the biological role of this novel receptor isoform. To this end, mRNA was collected from CD14 + cells separated from human PBMC by magnetic beads. First strand cDNA was synthesized with anchored primers and the expression profile of the 7TM and 5TM assessed by RT‐PCR, sequence analysis, and Southern hybridization. Our findings suggest preferential 5TM expression in blood monocytes (CD14 + ). Research supported by NIH‐KO1 1K01DK064828 and COBRE 2P20RR05566.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".