Screening <i>Legionella</i> effectors for antiviral effects reveals Rab1 GTPase as a proviral factor coopted for tombusvirus replication
Bibliographic record
Abstract
Significance Many bacterial pathogens infect eukaryotic cells by injecting bacterial virulence factors or effectors into the cytosol of their hosts. These effector proteins have evolved to manipulate cellular pathways and favor bacterial replication. Legionella pneumophila , the cause of Legionnaires’ disease, synthesizes >300 effector proteins. Here, we screened Legionella effectors to probe tombusvirus (TBSV)–host interactions and identify antiviral factors against TBSV in yeast surrogate host. As proof of concept that bacterial effectors can be used as tools to identify cellular pathways used by viruses, we characterized the antiviral effect of DrrA effector of Legionella . This led to the discovery that Rab1 small GTPase, the cellular target of DrrA, and COPII vesicles are coopted by TBSV for building the viral replication compartment.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".