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Record W2981906317 · doi:10.1101/818385

CCSeq: Clusters of Colocalized Sequences

2019· preprint· en· W2981906317 on OpenAlexfundno aff
Stefan Golas

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2019
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicNF-κB Signaling Pathways
Canadian institutionsnot available
FundersUniversity of AlbertaCompute Canada
KeywordsEnhancerComputational biologyGenomeDNA binding siteGeneticsTranscription factorBiologyAnnotationComputer sciencePromoterGene

Abstract

fetched live from OpenAlex

Abstract 0.1 Motivation Potential transcription factor (TF) complexes may be identified by testing whether the binding sequences of individual TF proteins form clusters with each other. These clusters may also indicate TF inhibition due to competitive occupancy of enhancer regions. Genome annotation data containing the coordinates of enhancer sequences is highly accessible via position-weight matrix tools. 0.2 Results An algorithm called CCSeq (Clusters of Colocalized Sequences) was developed for identifying clusters of sequences along a one-dimensional line, such as a chromosome, given genome annotation files and a cut-off distance as inputs. The algorithm was applied to the binding sequences of the constituent proteins of two known transcription factor complexes, the HSF1 homotrimer and one form of the NF- κ B complex, a dimer of NFKB2 and RELB. 28 clusters of HSF1 trimer binding sequences were identified on chromosome Y, and 16 clusters of the NFKB2 and RELB dimer were identified on chromosome 17, compared to 0 clusters identified in any of the five simulated random distributions for each of the two sets of TF proteins. Additionally, structural patterns of these binding sequence clusters are described. 0.3 Availability and Implementation This algorithm is freely available as an R package on the open source R repository CRAN at the following link: https://cran.r-project.org/package=colocalized . Genome annotation files were obtained from the PWMScan tool at https://ccg.epfl.ch/pwmtools/pwmscan.php hosted by the Swiss Insitute of Bioinformatics (2) (3).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.007
metaresearch head score (Gemma)0.023
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.075
Threshold uncertainty score0.251

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0070.023
Meta-epidemiology (narrow)0.0050.003
Meta-epidemiology (broad)0.0050.006
Bibliometrics0.0080.008
Science and technology studies0.0050.002
Scholarly communication0.0050.003
Open science0.0080.007
Research integrity0.0030.005
Insufficient payload (model declined to judge)0.0750.033

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.225
Teacher spread0.212 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2019
Admission routes1
Has abstractyes

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