Short‐term bed rest‐induced insulin resistance cannot be explained by increased mitochondrial H<sub>2</sub>O<sub>2</sub> emission
Bibliographic record
Abstract
Key points We determined if bed rest increased mitochondrially derived reactive oxygen species and cellular redox stress, contributing to the induction of insulin resistance. Bed rest decreased maximal and submaximal ADP‐stimulated mitochondrial respiration. Bed rest did not alter mitochondrial H 2 O 2 emission in the presence of ADP concentrations indicative of resting muscle, the ratio of H 2 O 2 emission to mitochondrial O 2 consumption or markers of oxidative stress The present data suggest strongly that mitochondrial H 2 O 2 does not contribute to bed rest‐induced insulin resistance Abstract Mitochondrial H 2 O 2 has been causally linked to diet‐induced insulin resistance, although it remains unclear if muscle disuse similarly increases mitochondrial H 2 O 2 . Therefore, we investigated the potential that an increase in skeletal muscle mitochondrial H 2 O 2 emission, potentially as a result of decreased ADP sensitivity, contributes to cellular redox stress and the induction of insulin resistance during short‐term bed rest in 20 healthy males. Bed rest led to a decline in glucose infusion rate during a hyperinsulinaemic‐euglycaemic clamp (−42 ± 2%; P < 0.001), and in permeabilized skeletal muscle fibres it decreased OXPHOS protein content (−16 ± 8%) and mitochondrial respiration across a range of ADP concentrations (−13 ± 5%). While bed rest tended to increase maximal mitochondrial H 2 O 2 emission rates ( P = 0.053), H 2 O 2 emission in the presence of ADP concentrations indicative of resting muscle, the ratio of H 2 O 2 emission to mitochondrial O 2 consumption, and markers of oxidative stress were not altered following bed rest. Altogether, while bed rest impairs mitochondrial ADP‐stimulated respiration, an increase in mitochondrial H 2 O 2 emission does not contribute to the induction of insulin resistance following short‐term bed rest.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".