Clostridium Difficile Infection Among Hematopoietic Stem Cell Transplant Recipients and Subsequent Development of Graft-Versus-Host Disease
Bibliographic record
Abstract
Introduction: Clostridium difficile (C. difficile) is an anaerobic gram positive rod that colonizes 20-40 % of hospitalized patients and the most common cause of antibiotic-associated diarrhea. The clinical manifestations of C. difficile infection (CDI) range from asymptomatic carriage to severe fulminant infection, leading to bowel perforation and death. Stem cell transplantation (SCT) recipients are at risk of CDI due to prolonged neutropenia, use of broad-spectrum antibiotics, mucosal damage and changes in intestinal microbiota induced by cytotoxic chemotherapy, microbial co-infection and possibly graft-versus-host disease (GVHD). The incidence of CDI amongst patients undergoing autologous SCT varies between five and 15 % and among those undergoing allogenic SCT 12 and 34% in literature1-5. Some studies have found a strong association between CDI and GVHD, gut GVHD and/or severe GVHD1-4 but this was not universally demonstrated5. Many studies looking at CDI in the SCT setting are limited by the ubiquity of traditional risk factors for CDI and small sample sizes. Methods: In this retrospective observational study, we aimed to review the epidemiology and clinical management of CDI in our autologous and allogeneic SCT recipients and subsequent clinical outcomes including development of GVHD. All recipients of autologous and allogeneic SCT at Vancouver General Hospital over the 10-year period between January 1, 2008 and December 31, 2017, who had diarrhoea and at least one positive stool test for C. difficile toxin by polymerase chain reaction were eligible for inclusion. Patient demographics, transplant details including GVHD prophylaxis, treatment for CDI, complications from CDI and subsequent development of acute and chronic GVHD and survival outcomes were collected by means of review of their electronic and written clinical records. An ethics approval was obtained from the hospital ethics committee. Results: Out of the 2104 patients who underwent SCT during the study period, 277 (13.2%) had at least one episode of C. difficile positive diarrhea. The incidence was higher among recipients of allogeneic SCT (19.6%) compared to autologous SCT recipients (9.6%). The majority of the patients (86.7%) were treated with oral metronidazole as a first line therapy and 18.9% required a second line treatment, with oral vancomycin in most cases, due to persistent symptoms with toxin positivity. 21% had recurrence of CDI after an initial successful treatment and 9.4% had two or more recurrences. There was no death directly attributable to acute CDI and the rate of major complications was low. Two patients required an intensive care unit admission with fulminant CDI and one patient underwent bowel resection. 57% of allogeneic SCT patients with post-transplant CDI subsequently developed acute GVHD (45.8% grade 2 or higher acute GVHD, 34% gut GVHD). 60.6% developed chronic GVHD (46.9% moderate to severe GVHD, 19.2% gut chronic GVHD). The median progression free survival was 670.5 days (range 1-3938) and the overall survival time was 984.5 days (range 10-3938). The commonest cause of death was malignancy relapse. Conclusion: The incidence of CDI among autologous and allogeneic SCT recipients in British Columbia was 9.6% and 19.6% respectively. There was no death immediately attributable to CDI and few patients developed fulminant CDI. Of the SCT patients who had CDI, 57% and 60.6% subsequently developed acute and chronic GVHD respectively. References: Trifilio SM et al . Changing epidemiology of Clostridium difficile-associated disease during stem cell transplantation. Biol Blood Marrow Transplant. 2013;19: 405-409.Chakrabarti et al.Clostridium difficile infection in allogeneic stem cell recipients is associated with severe graft-versus-host disease and non-relapse mortality. Bone Marrow Transplant. 2000; 26(8):871-6.Alonso CD et al. Epidemiology and outcomes of Clostridium difficile infections in hematopoietic stem cell transplant recipients. Clinical Infectious Diseases. 2012;54(8):1053-63.Dubberke ER et al. Clostridium difficile-associated disease in allogeneic hematopoietic stem cell transplant recipients: risk associations, protective associations, and outcomes. Clin Transplant. 2010;24(2):192-198.Kinnebrew MA et al. Early Clostridium difficile infection during allogeneic hematopoietic stem cell transplantation. Plos ONE 2014; 9(3):e90158. Disclosures No relevant conflicts of interest to declare.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".