The contribution of PIP2-type aquaporins to photosynthesis in response to increased vapour pressure deficit
Bibliographic record
Abstract
Abstract Roles of three different plasma membrane aquaporins (PIPs) in leaf-level gas exchange of Arabidopsis thaliana were examined using single, double and triple knockout mutants and compared to the Columbia-0 wild type (WT) plants. Since multiple Arabidopsis PIPs are implicated in conducting carbon dioxide across membranes, we focused on identifying whether the examined isoforms affect photosynthesis, either mediated through the control of stomatal conductance to water vapour ( g s ) or mesophyll conductance of CO 2 ( g m ) or a combination of both. In two separate studies, we grew Arabidopsis plants in a low humidity environment and under high humidity conditions. We found that the contribution of functional PIPs to g s was larger under conditions of low air humidity when the evaporative demand was high, whereas any effect of lacking PIP function was minimal under higher humidity conditions. The pip2;4 knockout mutants had 44% higher g s than the WT under low humidity conditions, which in turn resulted in an increased photosynthetic rate ( A net ). At PIP2;4 is thus likely to be involved in maintaining a positive water balance and high water use efficiency through mediation of transmembrane water flow. The lack of functional AtPIP2;5 on the other hand did not affect g s , but reduced g m indicating a possible role in regulating CO 2 membrane permeability. This potential regulatory function was indeed confirmed by subsequent stopped flow measurements of yeast expressing At PIP2;5.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".