Glyoxal/borate and RNase T1-based detection of inosine residues in RNA v1
Bibliographic record
Abstract
Adenosine-to-inosine (A-to-I) deamination is a functionally important modification of RNA that occurs in many metazoan nuclear transcripts, in several types tRNAs, and in mitochondrial transcripts of diplonemid protists. Several conceptually different methods exists to identify inosine residues in RNA,including the approach that exploits the cleavage after a guanosine or inosine residue by RNase T1. The digestion at Gs is blocked after an incubation in a glyoxal/borate solution and leads predominantly to cleavage 3′ to Is. This protocol blends the procedure originally conceived by Morse and Bass (DOI: 10.1021/bi9709607) and its more recent adaptation of Cattenoz et al. (DOI: 10.1261/rna.036202.112).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".