Morphological, cytological, and molecular variations induced by gamma rays in ground-grown chrysanthemum ‘Pinkling’
Why this work is in the frame
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Bibliographic record
Abstract
In this study, we analyzed the morphological, cytological, and molecular changes induced by different doses of 60 Co-γ radiation on root cuttings of the ground-grown chrysanthemum ‘Pinkling’ plants whose survival and height were decreased with increasing radiation dose. A variety of mutants were observed among irradiated ‘Pinkling’ plants; floral mutations included alteration of floral types, term ligulate floret number, capitulum diameter, and floral colour. Meiotic observation of mutants showed that the frequency of chromosome aberrations increased with radiation dose. The mutants were further analyzed using ISSR markers and on the basis of polymorphic DNA banding patterns, floral mutants were genotypically distinguished. Eighteen polymorphic ISSR primers produced 110 scorable bands, of which 93 (84.5%) were polymorphic. The percentage of polymorphism ranged from 60% to 100%. Jaccard’s dissimilarity coefficients varied from 0.337 to 1.000, which attested to the high genetic variability induced by irradiation. It is concluded that gamma ray irradiation induces a sufficient frequency of mutations and that ISSR analysis may offer a useful molecular marker for the detection of mutants in chrysanthemum. The present findings provide valuable information for future breeding of ‘Pinkling’.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it