Anillin/Mid1p interacts with the ECSRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast
Bibliographic record
Abstract
Abstract Cytokinesis is the final stage of the cell cycle which separates cellular constituents to produce two daughter cells. Using Schizosaccharomyces pombe we have investigated the role of various classes of proteins involved in this process. Central to these is anillin/Mid1p which forms a ring-like structure at the cell equator that predicts the site of cell separation through septation in fission yeast. Here we demonstrate a direct physical interaction between Mid1p and the endosomal sorting complex required for transport (ESCRT)-associated protein Vps4p. The interaction is essential for cell viability, and Vps4p is required for the correct cellular localization of Mid1p. Furthermore, we show that Mid1p is phosphorylated by the aurora kinase Aurora A, that the interaction of mid1 and ark1 genes is essential for cell viability, and that Ark1p is also required for the correct cellular localization of Mid1p. We mapped the sites of phosphorylation of Mid1p by Aurora A and the polo kinase Plk1 and assessed their importance by mutational analysis. Mutational analysis revealed S332, S523 and S531 to be required for Mid1p function and its interaction with Vps4p, Ark1p and Plo1p. Combined our data suggest a physical interaction between Mip1p and Vps4p important for cytokinesis, and identify phosphorylation of Mid1p by aurora and polo kinases as being significant for this process. Author summary Replication is a property of all living cells, with cell separation, so-called cytokinesis, the final step in the process. A large number of proteins have been identified that are required for cytokinesis, but in many cases it is not understand how they interact and regulate each other. In this research we have analysed two classes of proteins founds in all eukaryotic cells with central roles in cytokinesis: the endosomal sorting complex required for transport (ESCRT) proteins and the anillin protein Mid1p. We identify a direct physical interaction between the ESCRT protein Vps4 and anillin/Mid1p, and explore how it regulates cytokinesis. Midp1 activity is shown to controlled by the protein kinase Ark1p by direct phosphorylation, and this phosphorylation is important for Mid1p function. These observations identify new ways in which ESCRT and anillin/Mid1p control cell separation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".