Single-scan interferometric second harmonic generation microscopy using a kHz phase-scanner
Bibliographic record
Abstract
In conventional laser-scanning microscopy, images are formed by acquiring the signal from pixel to pixel. Here, we report more than one order of magnitude reduction in acquisition time of Interferometric Second Harmonic Generation (I-SHG) by scanning the phase within each pixel, to characterize the relative polarity of various samples. Using an electro-optic phase-scanner, we show that the phase-shift patterns required for interferometry can be applied at each pixel during the scanning of the sample, allowing single-scan I-SHG (1S-ISHG) measurements. Requiring exposure times comparable to standard SHG intensity images, the additional phase information of the signal can thus be retrieved in parallel to its amplitude at the time-scale of seconds. Moreover, slower modulations can be used to enhance the precision of the phase measurement, without any spatial or temporal shift between interferograms, in contrast to conventional frame phase-shifting I-SHG (standard I-SHG). This continues to extend I-SHG to dynamical processes, and opens it to large-scale studies, as well as to imaging samples where the signal-to-noise ratio is an issue.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".