Adapted Technological Platform for Screening and Identifying Compounds Capable of In vivo Spinal Network-mediated Reflex Ejaculation in Non-anesthetized, Chronic Paraplegic Mice: Evidence of Clonidine-elicited Seminal Emission
Bibliographic record
Abstract
Several drugs have been shown to facilitate locomotor rhythmogenesis and treadmill training after Spinal Cord Injury (SCI).Clonidine, an alpha-adrenoceptor agonist, is of particular interest given its well-known effects on facilitation of reflex-induced spinal stepping in acute or chronic complete lowthoracic spinal cord-transected (Tx) cats.Since locomotion and ejaculation are controlled by neuronal networks located in the same area of the spinal cord (i.e., upper-to mid-lumbar segments), we hypothesized that clonidine may have comparable effects on reflex ejaculation in Short Communication low-thoracic Tx animals.To achieve that, the main aim was to adapt a technological platform developed initially for in vivo testing of pro-locomotor compounds in order to establish and validate an approach for assessing both behaviourally and quantitatively, drug-induced reflex ejaculation ex copula in an animal model of SCI.At 6 or 7 days post-Tx, male mice received a single injection of clonidine (0.005-2.5 mg/kg, i.p.).At doses ranging between 0.1 and 2.5 mg/kg, clonidine acutely induced, in 88% of cases (35/40 mice), seminal emissions as fluids or plugs (in-urethra) with no erection nor expulsion.Given that clonidine is a partial alpha-2 agonist, the results suggest that alpha-2 adrenoceptor activation is associated with seminal emission in non-copulating and nonstimulated (e.g., manually or electrically) chronic paraplegic mice.To our knowledge, this is the first report of alpha-2-mediated, clonidine-induced seminal emission.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.005 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".