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Record W2997454792 · doi:10.1038/s41588-019-0559-8

The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins

2020· article· en· W2997454792 on OpenAlex
Kushal Suryamohan, Sajesh P Krishnankutty, Joseph Guillory, Matthew Jevit, Markus Schröder, Meng Wu, Boney Kuriakose, Oommen K. Mathew, Rajadurai Chinnasamy Perumal, Ivan Koludarov, Leonard D. Goldstein, Kate Senger, Mandumpala Davis Dixon, Dinesh Velayutham, Derek Vargas, Subhra Chaudhuri, Megha Muraleedharan, Ridhi Goel, Ying-Jiun J. Chen, Aakrosh Ratan, Peter Liu, Brendan K. Faherty, Guillermo de la Rosa, Hiroki Shibata, Miriam Baca, Meredith Sagolla, James Ziai, Gus A. Wright, Domagoj Vucic, Sangeetha Mohan, Aju Antony, Jeremy Stinson, Donald S. Kirkpatrick, Rami N. Hannoush, Steffen Durinck, Zora Modrušan, Eric Stawiski, Kristen Wiley, Terje Raudsepp, R. Manjunatha Kini, Arun Zachariah, Somasekar Seshagiri

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueNature Genetics · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicVenomous Animal Envenomation and Studies
Canadian institutionsUniversity of Toronto
FundersGenentech
KeywordsBiologyCobraTranscriptomeIdentification (biology)Computational biologyVenomGenomeGeneticsGeneGene expressionComputer scienceEcology

Abstract

fetched live from OpenAlex

Snakebite envenoming is a serious and neglected tropical disease that kills ~100,000 people annually. High-quality, genome-enabled comprehensive characterization of toxin genes will facilitate development of effective humanized recombinant antivenom. We report a de novo near-chromosomal genome assembly of Naja naja, the Indian cobra, a highly venomous, medically important snake. Our assembly has a scaffold N50 of 223.35 Mb, with 19 scaffolds containing 95% of the genome. Of the 23,248 predicted protein-coding genes, 12,346 venom-gland-expressed genes constitute the 'venom-ome' and this included 139 genes from 33 toxin families. Among the 139 toxin genes were 19 'venom-ome-specific toxins' (VSTs) that showed venom-gland-specific expression, and these probably encode the minimal core venom effector proteins. Synthetic venom reconstituted through recombinant VST expression will aid in the rapid development of safe and effective synthetic antivenom. Additionally, our genome could serve as a reference for snake genomes, support evolutionary studies and enable venom-driven drug discovery.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.761
Threshold uncertainty score0.299

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.251
Teacher spread0.234 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it