Resveratrol Significantly Attenuates Cell Mediated Immune Rejection Against Xenogenic Bovine Pericardium
Why this work is in the frame
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Bibliographic record
Abstract
Objective: Resveratrol is a bioactive phenol shown to reduce T cell activation through inhibition of mTOR pathways. Recent studies show cell-mediated immune reactions cause structural valve deterioration (SVD) in xenograft valves. Thus, tissue-engineered heart valves (TEHV) with decellularized bovine pericardium have been developed to reduce the immune response. Our study examines whether resveratrol could further decrease the immune rejection against xenogenic bovine pericardium. Methods: Human peripheral blood mononuclear cells (PBMC) were collected and isolated from patients undergoing cardiac surgery. The cells were further split into resveratrol (50μM/ml) and a vehicle group containing ethanol (50μM/ml). Culturing medium from each group containing 1 X 10^6 PBMC cells were then aliquoted into a cell culturing plate with each well containing either native bovine pericardium, decellularized bovine pericardium, allograft pericardium, autograft pericardium, or no pericardium. The exposed culture media were then collected for ELISA analysis for proinflammatory cytokine TNF-a quantification after 1 and 3 days. PBMC were further stained with proliferation dye and exposed to all tissue types for 3 days for flow cytometry to examine leukocyte proliferation. Results: We show that resveratrol treatment results in a significant reduction in all of the tissue exposure types in comparison to vehicle-treated controls at both 1 and 3 days (n=4, p<0.02). Importantly, resveratrol-treated native bovine pericardium exposure showed no significant difference in the TNF-a level when compared to the autologous human pericardial exposure group. Additionally, leukocyte proliferation decreased with the resveratrol treatment of xenogenic pericardial tissues. Conclusions: Resveratrol significantly decreases cell-mediated immune rejection against xenogenic bovine pericardium exposures. Therefore, resveratrol may serve as adjuvant therapy in TEHV replacements to further decrease the xenogenic immune reaction shown to cause SVD. KEYWORD: ICTEHV-O-21 The authors do not declare any conflict of interest.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it