A77 FURTHER CARACTERIZATION OF THE 67 KDA LAMININ RECEPTOR (67LR) IN COLORECTAL CANCER CELLS
Bibliographic record
Abstract
Abstract Background The 67 kDa laminin receptor (67LR) was the first non-integrin cell surface receptor for laminin isolated on laminin affinity columns from cancer cells in the 1980’s. Initially, 67LR is found as a cytoplasmic precursor of a 37 kDa protein, named 37LR, associated with the small ribosomal subunit. In human cells, 37LR allows the formation of the polyribosome complex and plays a key role in the initiation of translation. The mechanism by which the ribosomal protein becomes the 67LR membrane receptor is still unclear. It is presumed that the process involves post-translational modifications combined with homo or hetero-dimerization with non-associated ribosomal proteins. It has been shown that 37/67LR regulates adhesion and proliferation of normal human intestinal epithelial cells. Interestingly, overexpression of 37/67LR is correlated with aggressiveness and a poor prognosis in a wide variety of cancers. Aims The aim of this study was to confirm the overexpression of 37/67LR at the membrane of colorectal cancer cells and to identify its homo or heterodimerization partners. Methods To detect the expression of 37/67LR in colorectal cancer we performed an indirect immunofluorescence on tissues from normal and diseased colons. To confirm the presence of 67LR at the membrane of Caco-2 cells we used a cellular fractionation extraction protocol combined with ultracentrifugation and detergent treatment to separate ribosome-containing fractions from the membranes and isolate the membrane associated 67LR. Mass spectrometry analysis to study the molecular identity of 67LR was performed on immunoreactive bands corresponding to 37LR and 67LR. Results Immunolocalization of 37/67LR revealed an overexpression in colorectal cancer tissues. Following analysis by western blotting, immunoreactive 67LR protein was found in the soluble fraction after ultracentrifugation at 210,000 x g while 37LR was detected in the insoluble counterpart which was solubilized after treatment with detergent, suggesting that 37LR is associated with the membrane. Mass spectrometry analysis of these fractions indicated that 37LR was not identified in the immunoreactive bands of 67LR in the soluble fraction but identified the 67 kDa elastin binding protein, another 67 kDa cell surface laminin receptor. Conclusions These results indicate that 37LR is overexpressed in colorectal adenocarcinoma cells. Further characterization of the receptor by cell fractionation and mass spectrometry indicated that the 67 kDa immunoreactive form is not related to 37LR. Supported by CIHR. Funding Agencies CIHR
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".