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Record W3008352808 · doi:10.22215/etd/2014-10513

Novel Roles for Acyl-Synthetases and Acyl-Thioesterases in Plant Lipid Metabolism

2014· dissertation· en· W3008352808 on OpenAlexaff
Ian P. Pulsifer

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Surface Properties and Treatments
Canadian institutionsCarleton University
Fundersnot available
KeywordsArabidopsisBiochemistryThioesteraseCutinArabidopsis thalianaGalactolipidsBiologyEnzymeLipid metabolismAcyl-CoAChemistrySuberinBiosynthesisGeneCell wallChloroplast

Abstract

fetched live from OpenAlex

Acyl lipids are a diverse and important class of biomolecules.In plants, acyl lipids include ubiquitous compounds such as phospholipids, galactolipids, and triacylglycerols, as well as more specialized compounds including cutin and suberin monomers, cuticular waxes, and oxylipins.All acyl lipids are derived from fatty acids, which generally require activation for metabolism to either a CoenzymeA (CoA) or an acyl carrier protein (ACP) thioester.Acyl-synthetases and acyl-thioesterases, expressed heterologously in E. coli, each ALT protein generated a unique set of medium-chain fatty acids and/or medium-chain β-ketofatty acids.The occurrence of medium-chain fatty acids and β-ketofatty acids in various tissues of Arabidopsis plants provides clues regarding the biological roles of ALT1-4.Potential ALT homologues are found in nearly all plant genomes, and the encoded proteins are likely involved in specialized metabolism.This research opens many doors for future research, both in terms of basic research of plant lipid metabolism as well as applied uses of specialized high-value acyl lipids.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Other · Consensus signal: none
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0000.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.221
Teacher spread0.198 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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