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Record W3009357978 · doi:10.1038/s41597-019-0320-2

A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples

2019· article· en· W3009357978 on OpenAlexafffundabout
Jeremy R deWaard, Sujeevan Ratnasingham, Evgeny Zakharov, Alex Borisenko, Dirk Steinke, Angela C Telfer, Kate Perez, Jayme E Sones, Monica R Young, Valerie Levesque‐Beaudin, Crystal N Sobel, Arusyak Abrahamyan, Kyrylo Bessonov, Gergin Blagoev, Stephanie deWaard, Chris Ho, Natalya Ivanova, Kara K S Layton, Liuqiong Lu, Ramya Manjunath, Jaclyn McKeown, Megan Milton, Renee Miskie, Norm Monkhouse, Suresh Naik, Nadya Nikolova, Mikko Pentinsaari, Sean W. J. Prosser, Adriana Radulovici, Claudia Steinke, Connor P Warne, Paul D. N. Hebert

Bibliographic record

VenueScientific Data · 2019
Typearticle
Languageen
FieldEnvironmental Science
TopicEnvironmental DNA in Biodiversity Studies
Canadian institutionsDalhousie UniversityPublic Health Agency of CanadaUniversity of Guelph
FundersNature Conservancy of CanadaCanada First Research Excellence FundOntario GenomicsOntario Ministry of Research, Innovation and ScienceGovernment of CanadaChurchill Northern Studies CentreUniversity of GuelphMinistry of EnvironmentCanada Foundation for InnovationParks CanadaGenome CanadaGordon and Betty Moore FoundationNatural Sciences and Engineering Research Council of CanadaSmithsonian Institution
KeywordsGenBankBiodiversityBarcodeBiologyDNA barcodingTaxonomic rankGlobal biodiversityTaxonomy (biology)Environmental DNADNA sequencingZoologyEcologyGeographyTaxonDNAGeneticsComputer science

Abstract

fetched live from OpenAlex

The reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency, we provide DNA barcodes for 1,500,003 animal specimens collected from 23 terrestrial and aquatic ecozones at sites across Canada, a nation that comprises 7% of the planet's land surface. In total, 14 phyla, 43 classes, 163 orders, 1123 families, 6186 genera, and 64,264 Barcode Index Numbers (BINs; a proxy for species) are represented. Species-level taxonomy was available for 38% of the specimens, but higher proportions were assigned to a genus (69.5%) and a family (99.9%). Voucher specimens and DNA extracts are archived at the Centre for Biodiversity Genomics where they are available for further research. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, and the Global Genome Biodiversity Network Data Portal.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.005
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Dataset · Consensus signal: Dataset
Teacher disagreement score0.336
Threshold uncertainty score0.676

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.005
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0190.021
Science and technology studies0.0060.001
Scholarly communication0.0020.001
Open science0.0040.002
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0390.018

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.040
GPT teacher head0.208
Teacher spread0.169 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreDataset

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations110
Published2019
Admission routes3
Has abstractyes

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