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Record W3010631891 · doi:10.1101/2020.03.05.20032011

Amplicon based MinION sequencing of SARS-CoV-2 and metagenomic characterisation of nasopharyngeal swabs from patients with COVID-19

2020· preprint· en· W3010631891 on OpenAlex
Shona C. Moore, Rebekah Penrice-Randal, Muhannad Alruwaili, Xiaofeng Dong, Steven T. Pullan, Daniel Carter, Kevin R. Bewley, Qin Zhao, Yani Sun, Catherine Hartley, En‐Min Zhou, Tom Solomon, Michael Beadsworth, James Cruise, Debby Bogaert, Derrick W. Crook, David A. Matthews, Andrew D. Davidson, Zana H. Mahmood, Waleed Aljabr, Julian Druce, Richard Vipond, Lisa F. P. Ng, Laurent Rénia, Peter Openshaw, J. Kenneth Baillie, Miles W. Carroll, Malcolm G. Semple, Lance Turtle, Julian A. Hiscox

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenuemedRxiv · 2020
Typepreprint
Languageen
FieldMedicine
TopicRespiratory viral infections research
Canadian institutionsnot available
FundersMedical Research CouncilU.S. Food and Drug AdministrationPublic Health EnglandNational Institute for Health and Care ResearchNational Institute for Health Research Health Protection Research UnitHamilton Health Sciences FoundationKing Fahad Medical CityWellcome Trust
KeywordsMinionMetagenomicsAmpliconCoronavirus disease 2019 (COVID-19)MicrobiomeSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyBiologyAmplicon sequencing2019-20 coronavirus outbreakNanopore sequencingComputational biologyPandemicDNA sequencingMedicineGeneticsDiseaseGeneInfectious disease (medical specialty)Internal medicinePolymerase chain reactionOutbreak

Abstract

fetched live from OpenAlex

Abstract COVID-19 is a complex disease phenotype where the underlying microbiome could influence morbidity and mortality. Amplicon and metagenomic MinION based sequencing was used to rapidly (within 8 hours) identify SARS-CoV-2 and assess the microbiome in nasopharyngeal swabs obtained from patients with COVID-19 by the ISARIC 4C consortium.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.191
Threshold uncertainty score0.938

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.138
GPT teacher head0.369
Teacher spread0.231 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it