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Record W3015527317 · doi:10.1016/j.celrep.2020.03.075

Medulloblastoma Arises from the Persistence of a Rare and Transient Sox2+ Granule Neuron Precursor

2020· article· en· W3015527317 on OpenAlexafffund
Hayden Selvadurai, Erika Luis, Kinjal Desai, Xiaoyang Lan, Maria Vladoiu, Owen Whitley, Ciaran Galvin, Robert J. Vanner, Lilian Lee, Heather Whetstone, Michelle Kushida, Tomasz J. Nowakowski, Phedias Diamandis, Cynthia Hawkins, Gary D. Bader, Arnold R. Kriegstein, Michael D. Taylor, Peter B. Dirks

Bibliographic record

VenueCell Reports · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicHedgehog Signaling Pathway Studies
Canadian institutionsToronto General HospitalUniversity Health NetworkUniversity of TorontoSickKids FoundationHospital for Sick Children
FundersNational Institute of Mental HealthCanadian Institutes of Health ResearchNational Institutes of HealthSick Kids FoundationGenome Canada
KeywordsSonic hedgehogMedulloblastomaSOX2BiologyPrecursor cellPopulationCancer researchCell biologyNeuroscienceEmbryonic stem cellGeneticsCellMedicineSignal transductionGene

Abstract

fetched live from OpenAlex

Medulloblastoma (MB) is a neoplasm linked to dysregulated cerebellar development. Previously, we demonstrated that the Sonic Hedgehog (SHH) subgroup grows hierarchically, with Sox2 + cells at the apex of tumor progression and relapse. To test whether this mechanism is rooted in a normal developmental process, we studied the role of Sox2 in cerebellar development. We find that the external germinal layer (EGL) is derived from embryonic Sox2 + precursors and that the EGL maintains a rare fraction of Sox2 + cells during the first postnatal week. Through lineage tracing and single-cell analysis, we demonstrate that these Sox2 + cells are within the Atoh1 + lineage, contribute extensively to adult granule neurons, and resemble Sox2 + tumor cells. Critically, constitutive activation of the SHH pathway leads to their aberrant persistence in the EGL and rapid tumor onset. We propose that failure to eliminate this rare but potent developmental population is the tumor initiation mechanism in SHH-subgroup MB.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.209
Teacher spread0.189 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations57
Published2020
Admission routes2
Has abstractyes

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