Oncostatin M‐ Mediated Regulation of Matrix Metalloproteinases and Associated Mediators of MMPs' Expression in H‐ras Transformed Fibroblasts
Bibliographic record
Abstract
Oncostatin M (OSM) is a growth and differentiation factor which has been shown to exert both stimulatory and inhibitory effects on tissue invasion in various cell lines. Tissue invasion is a key marker of metastases and can be monitored by examining activities associated with ECM degradation such as matrix metalloproteinases (MMPs). This present study elucidates a link between OSM, MMPs, and various mediators associated with MMP activity. Treatment of NR3 cells (H‐ras transformed, benign tumor forming cells) with OSM resulted in a time and dose dependent induction of MMP‐9 and MMP‐2. MMP‐9 and MMP‐2 activity increased as early as 2 hours when exposed to OSM [200ng./ml]. Elevated MMP‐9 and MMP‐2 protein expression also occurred following treatment with OSM [50ng./ml] and this increase was maintained for 72 hours. The expression of other activities which affect MMP expression was determined in response to OSM treatment. EMMPRIN, RECK, TIMP‐1, and TIMP‐2 expression was determined. NR3 cells were exposed to OSM at 200ng./ml for 2 hours and 50ng./ml for 24 hours. Western blot analysis revealed that EMMPRIN protein expression increased dramatically, RECK protein expression decreased, TIMP‐1 protein expression increased, and TIMP‐2 showed no change at the protein expression level.These OSM‐mediated alterations in MMPs and related activities represent an aspect of the altered phenotype which occurs following H‐ras mediated cellular transformation of these cells. [N.S.E.R.C. & Canadian Cancer Society (P.E.I. Division)funded]
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".