Decreased mitochondrial enzyme content in adipose tissue after the consumption of a high fat diet is associated with oxidative stress
Bibliographic record
Abstract
Based upon studies in genetically modified rodents it has been suggested that decreases in mitochondrial content and/or activity in abdominal adipose tissue is involved in the pathogenesis of obesity and type II diabetes. It is currently not known if diet induced obesity results in similar changes and if so the mechanisms involved. The purpose of the present study was to examine the effects of diet induced obesity on mitochondrial enzyme content in mouse abdominal adipose tissue and determine if these changes are associated with the induction of oxidative stress and reductions in PGC‐1 content and AMPK activity. Male C57Bl/6 were fed a high fat (~60% kcals fat) diet for 12 weeks. High fat feeding resulted in increases in body weight and percent body fat. The mitochondrial marker enzymes CS, COX I and COXIV were reduced ~30‐50% in epididymal fat pads from fat versus chow fed mice. The content of PGC‐1, a regulator of mitochondrial biogenesis, was reduced ~60% in fat pads from high fat fed mice. The phosphorylation of AMPK, a reputed mediator of mitochondrial biogenesis was reduced ~40% independent of changes in total enzyme content. The consumption of a high fat diet led to increases in protein carbonyls in visceral adipose tissue, suggesting the induction of oxidative stress. Collectively, these findings suggest an association between oxidative stress, reductions in AMPK‐PGC‐1 signaling and decreases in mitochondrial marker enzyme content in a model of diet induced obesity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".