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Intra‐uterine Exposure to Diazepam Decrease the Ventilatory Response and Impairs the Motor Behavior of Males and Females at Post‐natal Life

2020· article· en· W3016451677 on OpenAlexaff
Carlos Aparecido da Silva, Vivian Biancardi, Luís Gustavo A. Patrone, Kênia C. Bícego, Luciane H. Gargaglioni

Bibliographic record

VenueThe FASEB Journal · 2020
Typearticle
Languageen
FieldNeuroscience
TopicNeuroscience of respiration and sleep
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsMedicineHypercapniaPlethysmographPregnancyAnesthesiaVentilation (architecture)Respiratory systemGestationTidal volumeRespiratory minute volumeHypoxia (environmental)ReflexPhysiologyInternal medicineBiologyChemistry

Abstract

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Gestation is a critical period for the development of the respiratory system and interventions, such as the use of benzodiazepines (BZs) for treatment of psychic disorders, at this stage, may compromise this system. BZs, such as Diazepam (DZ) are one of the most common drugs prescribed to treat anxiety disorders but it can compromise the development of the respiratory system during the gestational period since it crosses the placental barrier binding at GABA A receptors. Despite of your widely prescription, it is still unknown the real consequences of the administration DZ during pregnancy on breathing control during post‐natal life. We administered DZ (1mg/Kg) or its vehicle, via osmotic pump, to pregnant rats during whole pregnancy (21 day) to understand the consequences of intra‐uterine exposure to DZ on the ventilatory responses of newborns rats. We used newborns rats (male and female) at post‐natal (P) day 0 (P0), P12 and P21. After 4 hours of their birth we evaluated chewing reflex and straightening reflex. Ventilation (Ve), tidal volume (V T ), respiratory frequency (f R ) and oxygen consumption (VO2) were measured by using head‐out plethysmograph for P0 and P12 and using whole‐body plethysmograph for P21. Animals were exposed to normocapnic‐normoxic, hypercapnia (7% CO 2 ) and hypoxia (10% O 2 ). The project was approved by the local ethics committee: CEUA Protocol: 2164/17. DZ treatment of the dams decreased gestational length compared to control group but did not affect the number of male and female pups as well did not affect the total number of pups per litter. There are no differences on the average of number of chewing or on straightening reflex time although we have observed that the treated animals show less variability on chewing reflex compared with their same gander control group. At P21, both treated males and females have shown a higher body weight compared to their control group. Under room air conditions, P0 treated females shown a decrease in their f R and VO2, P12 treated males shown a reduction in their Ve due a reduction in their V T and P21 treated males have a decrease on Ve and it was due a reduction on their V T and they also have a reduction on their Ve/VO2. Under hypercapnia, P0 treated males have decrease in their f R , P0 treated females have a decrease in their Ve due a reduction in their V T , P12 treated males have a decrease on f R and VO2, P12 treated females have a decrease in their Ve due a reduction in their V T and a reduction in their Ve/VO2. Under hypoxia, P0 treated females show a decrease on V T , P12 treated animals have a decrease in their Ve. Intra‐uterine exposure to diazepam reduces male ventilation at baseline, which may lead the need to use vital support for maintain their correct oxygenation. Regard reduction on treated females’ ventilation under hypercapnic but not at baseline or hypoxia, we suggest that central chemosensitivity of this group is decreased. Support or Funding Information Financial Support: FAPESP (2017/26367‐9) and CNPq.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.041
GPT teacher head0.279
Teacher spread0.239 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2020
Admission routes1
Has abstractyes

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