Examination of Cortactin‐ and Membrane‐Associated Proteins at <i>Listeria</i> Membrane Protrusions
Bibliographic record
Abstract
Listeria monocytogenes is an intracellular pathogen that manipulates its host’s cytoskeleton to polymerize actin together with an array of actin‐associated proteins, including VASP and cortactin to one pole of the bacteria forming comet/rocket tails for intracellular propulsion. These structures also enable the microbes to move from one cell to another through a cell‐to‐cell spreading process involving the bacteria pressing into the host’s plasma membrane, forming L. monocytogenes protrusions which are internalized into corresponding invaginations in neighbouring cells. Recently, a siRNA screen listed genes involved in comet/rocket tail formation and cell‐to‐cell spreading. Here we examined a subset of those proteins (Endophilin, Ephrin A2, Anti‐Ephrin B1) as well as cortactin‐associated proteins (FGD1, SHK, VAV2) by immunolocalization at actin‐rich structures generated during L. monocytogenes infections.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".