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Comparative Neuromorphology of Purkinje Neurons Across Species

2020· article· en· W3016755997 on OpenAlexaffabout
Yi Fan Liu, Stefanie S. Bradley, Craig D. C. Bailey, Matthew K. Vickaryous

Bibliographic record

VenueThe FASEB Journal · 2020
Typearticle
Languageen
FieldNeuroscience
TopicNeural dynamics and brain function
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsBiologySomaGeckoEvolutionary biologyPurkinje cellCerebellumZoologyNeuroscience

Abstract

fetched live from OpenAlex

Neuromorphology of cells vary both across and within species, the differences of which contribute to a variety of cellular functions. Purkinje cells (PCs) in the cerebellum play a key role in the fine tuning of motor control and movement, and are characterized by their large soma and distinctive pattern of dendritic branching. However, outside of few selected taxa, the neuromorphology of PCs have not been well characterized. Here, we investigate PC neuromorphology in a representative reptile (leopard gecko), mammal (laboratory mouse), and bird (domestic chicken). Using a modified Golgi‐Cox protocol, differences in PC neuromorphology were quantified using Sholl and branched structure analyses. While PCs in all species demonstrated elaborate patterns of dendritic arborization, we quantified species‐specific differences in both cell size and branching complexity. The average dendritic length of PCs in geckos and mice were comparable, while those of chickens were almost twice as long. Chicken PCs are also characterized by a greater dendritic diameter and dendritic volume. Mouse and chicken PCs were significantly more complex than those of geckos, based on the number of dendritic terminals. Taken together, our findings demonstrate that even within the same cell type, there is considerable neuromorphological variation between species, potentially related to aspects of phylogeny, ecology, and functional morphology. Support or Funding Information Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grants 400358 to MKV and 2019‐04989 to CDCB

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.109
GPT teacher head0.300
Teacher spread0.191 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2020
Admission routes2
Has abstractyes

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