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A Comparison of the Effects of Several Agents Found in Common Foodstuffs on Cholecystokinin‐induced Tension in Guinea Pig Gallbladder Strips

2020· article· en· W3017240227 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueThe FASEB Journal · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBioactive natural compounds
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsChrysinLuteolinChemistryQuercetinCholecystokininPharmacologyResveratrolCurcuminGuinea pigFlavonoidBiochemistryEndocrinologyMedicineAntioxidant

Abstract

fetched live from OpenAlex

17beta‐estradiol (E2) has an inhibitory effect on the contractility of gastrointestinal smooth muscle, including the gallbladder. The inhibition of extracellular Ca 2+ entry mediated E2‐induced relaxation of either CCK‐ or KCl‐induced tension in male and female guinea pig gallbladder strips. Chrysin, an endocrine disrupter, and luteolin are flavones. Resveratrol is a non‐flavonoid phytoestrogen, quercetin a phytoestrogen, and curcumin a diarylheptanoid. All are natural constituents of common foodstuffs. All relaxed CCK‐induced tension in male guinea pig gallbladder strips. Luteolin, quercetin, curcumin, resveratrol, and chrysin relaxed the CCK‐induced tension in the gallbladder strips by blocking extracellular Ca 2+ entry and intracellular Ca 2+ release. The effect of luteolin was also mediated by the PKA/cAMP system while quercetin also involved NO. In addition to the Ca 2+ effects, the effects of curcumin and chrysin involved the PKC system. The purpose of this study was to determine which of these agents was most potent in relaxing the CCK‐induced tension in gallbladder strips. Luteolin was the most potent (98.2±2.0%), chrysin (45.4±3.7%), resveratrol (41.1±4.0%), quercetin (42.9±3.0%), curcumin (39.4±4.0%), and E2 (64.5±2.3%) when using 50 mM of each agent. Luteolin had the largest effect at all the other concentrations used (10, 25, 50, and 100 mM) in relaxing CCK‐induced tension. Curcumin was the least potent. There was no significant difference in the amount of relaxation induced by chrysin, resveratrol, and quercetin were compared. The effect of E2 was second only to luteolin. Support or Funding Information This research did not receive any specific grant from funding agencies in the public, commercial, or not‐for‐profit sectors.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.194
Threshold uncertainty score0.358

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.310
Teacher spread0.274 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it