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Record W3018320276 · doi:10.1101/2020.04.19.20071373

Scalable and Resilient SARS-CoV-2 testing in an Academic Centre

2020· preprint· en· W3018320276 on OpenAlexaff
R. John Aitken, Karen Ambrose, Sam Barrell, Rupert Beale, Ganka Bineva‐Todd, Dhruva Biswas, Richard Byrne, Simon Caidan, Peter Cherepanov, Laura Churchward, G.M.G. Clark, Margaret Crawford, Laura Cubitt, Vicky Dearing, Christopher Earl, Alexandra Edwards, Chris Ekin, Efthymios Fidanis, Alessandra Gaiba, S.J. Gamblin, Sonia Gandhi, Jennifer L. Goldman, Robert Goldstone, PR Grant, Maria Greco, Judith Heaney, Steve Hindmarsh, Catherine Houlihan, Michael Howell, Michael Hubank, Deborah Hughes, R Instrell, D.A. Jackson, Mariam Jamal‐Hanjani, Ming Jiang, Mark Johnson, Lewis Jones, Nnennaya Kanu, George Kassiotis, Stuart A. Kirk, Svend Kjær, Andrew Levett, Lisa J. Levett, Marcel Levi, Wei-Ting Lu, James I. MacRae, J. B. Matthews, Laura E. McCoy, Catherine Moore, David Moore, Eleni Nastouli, Jérôme Nicod, Luke Nightingale, Jessica Olsen, Nicola O’Reilly, Amit Pabari, Venizelos Papayannopoulos, Nishith Patel, Nicola Peat, Michael G. Pollitt, Peter J. Ratcliffe, Sousa C Reis e, Annachiara Rosa, Rachel Rosenthal, Chloë Roustan, Andrew Rowan, GY Shin, Daniel M. Snell, O-R Song, Moira Spyer, A. Strange, Charles Swanton, JMA Turner, Maria L. Turner, Andreas Wack, Peter A. Walker, Sophia Ward, WK Wong, J. D. Wright, Mary Wu

Bibliographic record

VenuemedRxiv · 2020
Typepreprint
Languageen
FieldMedicine
TopicSARS-CoV-2 detection and testing
Canadian institutionsRoyal Ottawa Mental Health Centre
FundersMedical Research CouncilHorizon 2020 Framework ProgrammeNational Institute for Health and Care ResearchWellcome TrustFrancis Crick InstituteCancer Research UKUniversity College London Hospitals NHS Foundation TrustBreast Cancer Research Foundation
KeywordsBespokeTurnaround timePandemicSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Economic shortagePersonal protective equipmentHealth careMedical emergencyComputer scienceVirologyBusinessMedicineOperations managementEngineeringPathologyInfectious disease (medical specialty)

Abstract

fetched live from OpenAlex

Abstract The emergence of the novel coronavirus SARS-CoV-2 has led to a pandemic infecting more than two million people worldwide in less than four months, posing a major threat to healthcare systems. This is compounded by the shortage of available tests causing numerous healthcare workers to unnecessarily self-isolate. We provide a roadmap instructing how a research institute can be repurposed in the midst of this crisis, in collaboration with partner hospitals and an established diagnostic laboratory, harnessing existing expertise in virus handling, robotics, PCR, and data science to derive a rapid, high throughput diagnostic testing pipeline for detecting SARS-CoV-2 in patients with suspected COVID-19. The pipeline is used to detect SARS-CoV-2 from combined nose-throat swabs and endotracheal secretions/ bronchoalveolar lavage fluid. Notably, it relies on a series of in-house buffers for virus inactivation and the extraction of viral RNA, thereby reducing the dependency on commercial suppliers at times of global shortage. We use a commercial RT-PCR assay, from BGI, and results are reported with a bespoke online web application that integrates with the healthcare digital system. This strategy facilitates the remote reporting of thousands of samples a day with a turnaround time of under 24 hours, universally applicable to laboratories worldwide.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.003
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.296
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.003
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.002
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.115
GPT teacher head0.348
Teacher spread0.233 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations14
Published2020
Admission routes1
Has abstractyes

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