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Record W3020360613 · doi:10.1038/s41467-020-15743-6

Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice

2020· article· en· W3020360613 on OpenAlexafffund
Julia Duerr, Dominik Leitz, Magdalena Szczygieł, Dmytro Dvornikov, Simon G. Fraumann, Clemens Kreutz, Piotr K. Zadora, Ayça Seyhan Agircan, Philip Konietzke, Theresa A. Engelmann, Jan Hegermann, Surafel Mulugeta, Hiroshi Kawabe, Lars Knudsen, Matthias Ochs, Daniela Rotin, Thomas Muley, Michael Kreuter, Felix Herth, Mark O. Wielpütz, Michael F. Beers, Ursula Klingmüller, Marcus Mall

Bibliographic record

VenueNature Communications · 2020
Typearticle
Languageen
FieldMedicine
TopicInterstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Canadian institutionsSickKids FoundationHospital for Sick ChildrenUniversity of Toronto
FundersNational Institute of Environmental Health SciencesNational Heart, Lung, and Blood InstituteCanadian Institutes of Health ResearchNational Institutes of HealthBundesministerium für Bildung und ForschungUniversität HeidelbergEuropean Molecular Biology LaboratoryDeutsche ForschungsgemeinschaftGovernment of CanadaCincinnati Children's Hospital Medical CenterUniversity of PennsylvaniaCanada Foundation for InnovationPulmonary Fibrosis FoundationDeutsches KrebsforschungszentrumEinstein Stiftung BerlinU.S. Department of Veterans Affairs
KeywordsIdiopathic pulmonary fibrosisHoneycombingPirfenidoneLungMedicinePathogenesisPulmonary fibrosisFibrosisInterstitial lung diseasePathologyUbiquitin ligaseCancer researchImmunologyUbiquitinBiologyInternal medicine

Abstract

fetched live from OpenAlex

Abstract Idiopathic pulmonary fibrosis (IPF) is a chronic progressive interstitial lung disease characterized by patchy scarring of the distal lung with limited therapeutic options and poor prognosis. Here, we show that conditional deletion of the ubiquitin ligase Nedd4-2 ( Nedd4l ) in lung epithelial cells in adult mice produces chronic lung disease sharing key features with IPF including progressive fibrosis and bronchiolization with increased expression of Muc5b in peripheral airways, honeycombing and characteristic alterations in the lung proteome. NEDD4-2 is implicated in the regulation of the epithelial Na + channel critical for proper airway surface hydration and mucus clearance and the regulation of TGFβ signaling, which promotes fibrotic remodeling. Our data support a role of mucociliary dysfunction and aberrant epithelial pro-fibrotic response in the multifactorial disease pathogenesis. Further, treatment with the anti-fibrotic drug pirfenidone reduced pulmonary fibrosis in this model. This model may therefore aid studies of the pathogenesis and therapy of IPF.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.288
Teacher spread0.276 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations108
Published2020
Admission routes2
Has abstractyes

Explore more

Same venueNature CommunicationsSame topicInterstitial Lung Diseases and Idiopathic Pulmonary FibrosisFrench-language works237,207