Molecularly Imprinted Electrochemical Sensor for Naloxone Detection

Introduction Naloxone, (5α)-4,5-epoxy,3,14-dihydroxy17(2-propenyl) morphinan-6-one, (Figure 1) is a synthetic opioid receptor antagonist mainly used for the treatment of opioid overdose and to reduce constipation caused by orally administered opioid therapy [1]. A number of analytical methods have been reported for the detection of naloxone, mainly by high performance liquid chromatography [2], high performance liquid chromatography coupled with mass spectrometry [3] and chemiluminescence [4]. However, the methods are costly, time consuming, produce large amounts of liquid waste which is not environment friendly, and not appropriate for field use. Electrochemical sensor platforms, however, are attractive for handheld detection and field use due to their low sample volume requirement, simplicity and compactness. In this work, we report on the development of simple, yet sensitive and selective electrochemical sensor for naloxone detection using molecular imprinted polymer (MIP) and screen printing electrodes. Method The MIP preparation was carried out via in situ electropolymerization of a solution composed of the functional monomer, p-phenylenediamine (pPD), and the template (naloxone) in phosphate citrate buffer at pH 6 on a screen printed carbon electrode that was modified with reduced graphene oxide (rGO) and gold nanoparticle (AuNPs) (Figure 2). Several parameters controlling the preparation and performance of the MIP sensor (including pH, the molar ratio between monomer and template molecules, the cycle number of electropolymerization, and incubation time of the modified electrode on the sensing performance) were studied and optimized. After electropolymerization, naloxone molecules were removed from the MIP using methanol/HCl solution to generate binding sites that were complimentary in size, shape and functionality to naloxone molecules for later detection. Non-imprinted polymer (NIP) modified electrodes were prepared using the optimized procedure but in the absence of naloxone to examine the selectivity of the MIP sensor. The electrochemical behavior of naloxone at MIP and NIP sensors was evaluated by differential pulse voltammetry. Results and Conclusions The morphology and properties of the sensing material were characterized with scanning electron microscopy, Raman spectroscopy, and atomic force microscope. Under an optimized condition, the MIP electrochemical sensor responded linearly to naloxone concentration between 0.5 μM to 8 μM, with a detection limit of 0.23 μM. The introduction of rGO and AuNPs hybrid materials significantly improved the sensor’s performance. The selectivity of the MIP sensor towards naloxone was examined using morphine, naltrexone and noroxymorphone as interferents. The result of the selectivity experiment showed that the imprinted electrode has a good response and selectivity towards naloxone. To further demonstrate the potential of the developed MIP-based naloxone sensor for practical applications, the sensor was tested for the detection of naloxone in spiked urine samples. Recoveries of up to 97.0% were recorded, demonstrating the reliability and accuracy of the sensor for naloxone detection in bodily fluids. References [1] M. Liu and E. Wittbrodt, J. Pain Symptoms Manage, 2002, 23, 48-53. [2] D. A. Michels, M. Parker and O. Salas-Solano, Electrophoresis, 2012, 33, 815–826. [3] R. M. Vicente, N. Z. Fernández et al, J. Pharmaceutical and Biomedical Analysis, 2015, 114, 105–112. [4] N. A. Alarfaj, and M. F. El-Tohamy, Chemistry Central Journal, 2015, 9, 1-9. Figure 1

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.