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Record W3026373931 · doi:10.1038/s42003-019-0721-y

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

2019· article· en· W3026373931 on OpenAlexafffund
A.G. Hettle, Joanne K. Hobbs, B. Pluvinage, Chelsea Vickers, Kento T. Abe, Orly Salama-Alber, Bailey E. McGuire, Jan‐Hendrik Hehemann, Joseph P. M. Hui, Fabrice Berrué, Arjun H. Banskota, Junzeng Zhang, Eric Bottos, Jonathan D. Van Hamme, A.B. Boraston

Bibliographic record

VenueCommunications Biology · 2019
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicSeaweed-derived Bioactive Compounds
Canadian institutionsThompson Rivers UniversityNational Research Council CanadaLunenfeld-Tanenbaum Research InstituteUniversity of Victoria
FundersSLAC National Accelerator LaboratoryBiological and Environmental ResearchNational Research Council CanadaWestern Economic Diversification CanadaBasic Energy SciencesCanadian Light SourceNatural Sciences and Engineering Research Council of CanadaBC Cancer AgencyOffice of ScienceCanadian Institutes of Health ResearchNational Institute of General Medical SciencesCanada's Michael Smith Genome Sciences CentreNational Institutes of HealthUniversity of SaskatchewanU.S. Department of Energy
KeywordsPseudoalteromonasCarrageenanMetabolismChemistryBiologyBiochemistryGene

Abstract

fetched live from OpenAlex

is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.035
GPT teacher head0.248
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations70
Published2019
Admission routes2
Has abstractyes

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