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Record W3027341723 · doi:10.1101/gr.250704.119

Comprehensive analyses of 723 transcriptomes enhance genetic and biological interpretations for complex traits in cattle

2020· article· en· W3027341723 on OpenAlex
Lingzhao Fang, Wentao Cai, Shuli Liu, Oriol Canela‐Xandri, Yahui Gao, Jicai Jiang, Konrad Rawlik, Bingjie Li, Steven Schroeder, Benjamin D. Rosen, Congjun Li, Tad S. Sonstegard, Leeson J. Alexander, Curtis P. Van Tassell, P.M. VanRaden, John B. Cole, Ying Yu, Shengli Zhang, Albert Tenesa, Li Ma, George E. Liu

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueGenome Research · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenetic and phenotypic traits in livestock
Canadian institutionsnot available
FundersBiotechnology and Biological Sciences Research CouncilAgricultural Research ServiceOak Ridge Institute for Science and EducationNational Institute of Food and AgricultureMedical Research Council CanadaU.S. Department of AgricultureUnited States - Israel Binational Agricultural Research and Development FundMedical Research CouncilDirectorate for Biological SciencesU.S. Department of Energy
KeywordsBiologyTranscriptomeGeneGeneticsEpigenomicsComputational biologyGenomeDNA methylationQuantitative trait locusRNA-SeqGenome-wide association studySomatic cellEvolutionary biologyGene expressionSingle-nucleotide polymorphism

Abstract

fetched live from OpenAlex

By uniformly analyzing 723 RNA-seq data from 91 tissues and cell types, we built a comprehensive gene atlas and studied tissue specificity of genes in cattle. We demonstrated that tissue-specific genes significantly reflected the tissue-relevant biology, showing distinct promoter methylation and evolution patterns (e.g., brain-specific genes evolve slowest, whereas testis-specific genes evolve fastest). Through integrative analyses of those tissue-specific genes with large-scale genome-wide association studies, we detected relevant tissues/cell types and candidate genes for 45 economically important traits in cattle, including blood/immune system (e.g., CCDC88C ) for male fertility, brain (e.g., TRIM46 and RAB6A ) for milk production, and multiple growth-related tissues (e.g., FGF6 and CCND2 ) for body conformation. We validated these findings by using epigenomic data across major somatic tissues and sperm. Collectively, our findings provided novel insights into the genetic and biological mechanisms underlying complex traits in cattle, and our transcriptome atlas can serve as a primary source for biological interpretation, functional validation, studies of adaptive evolution, and genomic improvement in livestock.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.929
Threshold uncertainty score0.350

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.212
GPT teacher head0.425
Teacher spread0.213 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it