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Record W3030759028 · doi:10.15454/tombyz

Diat.barcode, an open-access barcode library for diatoms

2018· dataset· en· W3030759028 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueRecherche Data Gouv France · 2018
Typedataset
Languageen
FieldEnvironmental Science
TopicEnvironmental DNA in Biodiversity Studies
Canadian institutionsContinental (Canada)
Fundersnot available
KeywordsBarcodeDNA barcodingIdentification (biology)World Wide WebDNA sequencingComputer scienceBiologyDatabaseLibrary scienceData scienceEcologyGene

Abstract

fetched live from OpenAlex

Diatoms (Bacillariophyta) are ubiquitous microalgae which produce a siliceous exoskeleton and which make a major contribution to the productivity of oceans and freshwaters. They display a huge diversity, which makes them excellent ecological indicators of aquatic ecosystems, and can also be used to reconstruct paleoenvironments. Usually, diatoms are identified using characteristics of their exoskeleton morphology, which can be time consuming and error-prone. DNA-barcoding is an alternative to this and the use of High-Throughput-Sequencing enables the rapid analysis of many environmental samples at a lower cost than if specialist analysts are used. However, to identify environmental sequences correctly, an expertly curated reference library is needed. Several curated libraries for protists exists; none, however, are dedicated to diatoms. Diat.barcode is an open-access library dedicated to diatoms which has been maintained since 2012. It was initiated with the barcoding network of INRA (French National Institute for Agricultural Research) R-Syst, is now an international initiative partly supported by a Cost network (DNAqua-net). Data come from two sources (1) the NCBI nucleotide database (National Center for Biotechnology Information) and (2) unpublished sequencing data of culture collections in France, UK and Russia. Since 2017, several European experts have collaborated to curate this library for rbcL, a chloroplast marker suitable for species-level identification of diatoms. For the latests versions of the database, more than 8100 curated barcodes are available. The database is accessible through https://eng-carrtel-collection.hub.inrae.fr/barcoding-databases. A ready-to-use subset of the database for metabarcoding analyses is also accessible.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Open science, Insufficient payload (model declined to judge)
Consensus categoriesOpen science, Insufficient payload (model declined to judge)
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Dataset · Consensus signal: Dataset
Teacher disagreement score0.049
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0010.001
Scholarly communication0.0010.008
Open science0.0220.036
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0060.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.288
GPT teacher head0.415
Teacher spread0.126 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it