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Record W3032374457 · doi:10.1073/pnas.2002004117

Targeted mutation of secretogranin-2 disrupts sexual behavior and reproduction in zebrafish

2020· article· en· W3032374457 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2020
Typearticle
Languageen
FieldMedicine
TopicHypothalamic control of reproductive hormones
Canadian institutionsUniversity of Ottawa
FundersChinese Academy of SciencesNatural Sciences and Engineering Research Council of CanadaGovernment of Canada
KeywordsZebrafishBiologyMutantCell biologyEmbryoSexual reproductionGeneGenetics

Abstract

fetched live from OpenAlex

Significance The secretory granule protein secretogranin-2 is processed to the bioactive neuropeptide secretoneurin. While these are expressed in hypothalamic neurons and anterior pituitary cells, a role in hormonal control of reproduction is unknown. We provide in vivo evidence that secretogranin-2 plays critical roles in fertility regulation. Mutation of the secretogranin-2a and -2b genes in zebrafish leads to disrupted sexual behaviors, reduced ovulation and egg laying, and suboptimal fertility and embryo survival. Injection of a synthetic secretoneurin peptide enhanced reproductive outcomes in the mutant fish. Our secretogranin-2 mutants offer an amenable model system in which to screen potential injectable stimulators of reproduction, be they for spawning in cultured fish species, or to help with the search for new human infertility treatments.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.570
Threshold uncertainty score0.228

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.309
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it