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Record W3033763368 · doi:10.1128/msphere.00293-20

Combining Whole-Genome Sequencing and Multimodel Phenotyping To Identify Genetic Predictors of <i>Salmonella</i> Virulence

2020· article· en· W3033763368 on OpenAlex
Alanna Crouse, Catherine Schramm, Jean-Guillaume Emond-Rhéault, Adrian Herod, Maud Kerhoas, John R. Rohde, Samantha Gruenheid, Irena Kukavica‐Ibrulj, Brian Boyle, Celia M.T. Greenwood, Lawrence Goodridge, Rafael A. Garduño, Roger C. Lévesque, Danielle Malo, France Daigle

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenuemSphere · 2020
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicSalmonella and Campylobacter epidemiology
Canadian institutionsCanadian Food Inspection AgencyFonds de Recherche du Québec – Nature et TechnologiesUniversity of GuelphUniversité LavalUniversité de MontréalDalhousie UniversityJewish General HospitalMcGill University
FundersGenome Canada
KeywordsVirulenceSalmonellaSalmonella entericaBiologySerotypeGenomicsWhole genome sequencingGenomeFood safetyGenetic diversityGeneticsMicrobiologyBacteriaGenePopulationMedicineFood scienceEnvironmental health

Abstract

fetched live from OpenAlex

Salmonella species are bacteria that are a major source of foodborne disease through contamination of a diversity of foods, including meat, eggs, fruits, nuts, and vegetables. More than 2,600 different Salmonella enterica serovars have been identified, and only a few of them are associated with illness in humans. Despite the fact that they are genetically closely related, there is enormous variation in the virulence of different isolates of Salmonella enterica . Identification of foodborne pathogens is a lengthy process based on microbiological, biochemical, and immunological methods. Here, we worked toward new ways of integrating whole-genome sequencing (WGS) approaches into food safety practices. We used WGS to build associations between virulence and genetic diversity within 83 Salmonella isolates representing 77 different Salmonella serovars. Our work demonstrates the potential of combining a genomics approach and virulence tests to improve the diagnostics and assess risk of human illness associated with specific Salmonella isolates.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.903
Threshold uncertainty score0.320

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.041
GPT teacher head0.248
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it