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Record W3034718679 · doi:10.1038/s42003-020-1042-x

Chemogenomic profiling of breast cancer patient-derived xenografts reveals targetable vulnerabilities for difficult-to-treat tumors

2020· article· en· W3034718679 on OpenAlex
Paul Savage, Alain Pacis, Hellen Kuasne, Leah Liu, Daniel Lai, Adrian Wan, Matthew Dankner, Constanza Martínez, Valentina Muñoz-Ramos, Virginie Pilon, Anie Monast, Hong Zhao, Margarita Souleimanova, Matthew G. Annis, Adriana Aguilar‐Mahecha, Josiane Lafleur, Nicholas Bertos, Jamil Asselah, Nathaniel Bouganim, Kevin Petrecca, Peter M. Siegel, Atilla Ömeroğlu, Sohrab P. Shah, Samuel Aparício, Mark Basik, Sarkis Meterissian, Morag Park

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueCommunications Biology · 2020
Typearticle
Languageen
FieldMedicine
TopicCancer Cells and Metastasis
Canadian institutionsJewish General HospitalMcGill University and Génome Québec Innovation CentreUniversity of British ColumbiaMcGill UniversityMcGill University Health Centre
FundersFonds de Recherche du Québec - SantéMcGill UniversityStand Up To CancerUniversity of Texas MD Anderson Cancer CenterCanadian Institutes of Health ResearchMcGill University Health CentreNational Cancer InstituteGovernment of Canada
KeywordsBreast cancerTranscriptomeMedicineCancer researchXenotransplantationIn vivoMetastatic breast cancerBioinformaticsCancerOncologyBiologyInternal medicineGeneTransplantationGene expressionGenetics

Abstract

fetched live from OpenAlex

Subsets of breast tumors present major clinical challenges, including triple-negative, metastatic/recurrent disease and rare histologies. Here, we developed 37 patient-derived xenografts (PDX) from these difficult-to-treat cancers to interrogate their molecular composition and functional biology. Whole-genome and transcriptome sequencing and reverse-phase protein arrays revealed that PDXs conserve the molecular landscape of their corresponding patient tumors. Metastatic potential varied between PDXs, where low-penetrance lung micrometastases were most common, though a subset of models displayed high rates of dissemination in organotropic or diffuse patterns consistent with what was observed clinically. Chemosensitivity profiling was performed in vivo with standard-of-care agents, where multi-drug chemoresistance was retained upon xenotransplantation. Consolidating chemogenomic data identified actionable features in the majority of PDXs, and marked regressions were observed in a subset that was evaluated in vivo. Together, this clinically-annotated PDX library with comprehensive molecular and phenotypic profiling serves as a resource for preclinical studies on difficult-to-treat breast tumors.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.056
Threshold uncertainty score0.445

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.054
GPT teacher head0.325
Teacher spread0.271 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it