Highly Multiplexed Confocal Fluorescence Lifetime Microscope Designed for Screening Applications
Bibliographic record
Abstract
Protein-protein interactions can be measured in live cells, at nanometer scale, using Fluorescence Lifetime Imaging Microscopy (FLIM) enabled Forster Resonance Energy Transfer (FRET). There are growing interests in exploring protein-protein interactions in drug discovery applications. Traditional single point confocal microscopes, however, are slow and unsuited to small molecule screening, especially when combined with FLIM-FRET. We developed a 32 × 32 multiplexed confocal microscope, which employs a single-photon avalanche photodiode array with time gating capabilities for rapid FLIM acquisition. It has been demonstrated that such multiplexing technique can capture a 960 × 960 pixel multi-channel confocal fluorescence lifetime images in less than 1.5 seconds. Binding curves of two Bcl-2 family proteins: Bcl-XL and Bad were generated in live cells imaging experiments. The results show that the small molecule inhibitor A-1131852 is a more effective compound for disrupting Bcl-XL binding to Bad than ABT-263, which demonstrated the feasibility of screening of protein-protein interactions in high density well-plates.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".