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Methods developed during the first National Center for Biotechnology Information Structural Variation Codeathon at Baylor College of Medicine

2020· preprint· en· W3085310600 on OpenAlexfundno aff
Medhat Mahmoud, Alejandro R. Gener, Michael M. Khayat, Adam C. English, Advait Balaji, Anbo Zhou, Andreas Hehn, Arkarachai Fungtammasan, Brianna Chrisman, Chen-Shan Chin, Chiao‐Feng Lin, Chun-Hsuan Lo, Chunxiao Liao, Claudia M.B. Carvalho, Colin Diesh, David E. Symer, Divya Kalra, Dreycey Albin, Elbay Aliyev, Eric T. Dawson, Eric Venner, Fernanda Foertter, Gigon Bae, Haowei Du, Joyjit Daw, Junzhou Wang, Keiko Akagi, Lon Phan, Michael D. Jochum, Mohammadamin Edrisi, Nirav N. Shah, Qi Wang, Robert Fullem, Rong Zheng, Sara E. Kalla, Shakuntala Mitra, Todd J. Treangen, Vaidhyanathan Mahaganapathy, Venkat S. Malladi, Vipin K. Menon, Yilei Fu, Yongze Yin, Yuanqing Feng, Tim Hefferon, Fritz J. Sedlazeck, Ben Busby

Bibliographic record

VenueF1000Research · 2020
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsnot available
FundersInstitute of GeneticsEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentU.S. National Library of MedicineNational Institute of Neurological Disorders and StrokeNational Institute of General Medical SciencesU.S. Department of Health and Human ServicesNational Institutes of HealthNational Cancer InstituteOxford Nanopore TechnologiesRice University
KeywordsMetagenomicsAnnotationPopulationGenomeCopy-number variationComputational biologyBiologyLibrary scienceMedicineBioinformaticsComputer scienceGeneticsGene

Abstract

fetched live from OpenAlex

<ns3:p> In October 2019, 46 scientists from around the world participated in the first National Center for Biotechnology Information (NCBI) Structural Variation (SV) Codeathon at Baylor College of Medicine. The charge of this first annual working session was to identify ongoing challenges around the topics of SV and graph genomes, and in response to design reliable methods to facilitate their study. Over three days, seven working groups each designed and developed new open-sourced methods to improve the bioinformatic analysis of genomic SVs represented in next-generation sequencing (NGS) data. The groups’ approaches addressed a wide range of problems in SV detection and analysis, including quality control (QC) assessments of metagenome assemblies and population-scale VCF files, <ns3:italic>de novo</ns3:italic> copy number variation (CNV) detection based on continuous long sequence reads, the representation of sequence variation using graph genomes, and the development of an SV annotation pipeline. A summary of the questions and developments that arose during the daily discussions between groups is outlined. The new methods are publicly available at <ns3:ext-link xmlns:ns4="http://www.w3.org/1999/xlink" ext-link-type="uri" ns4:href="https://github.com/NCBI-Codeathons/MASQ">https://github.com/NCBI-Codeathons/</ns3:ext-link> , and demonstrate that a codeathon devoted to SV analysis can produce valuable new insights both for participants and for the broader research community. </ns3:p>

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.550
Threshold uncertainty score0.534

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.002
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.048
GPT teacher head0.362
Teacher spread0.314 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2020
Admission routes1
Has abstractyes

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