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Record W3089507664

Sex differences in neutrophil extracellular trap formation

2018· dissertation· en· W3089507664 on OpenAlexfundno aff
Xi Zhou Yuan

Bibliographic record

VenueTSpace (University of Toronto) · 2018
Typedissertation
Languageen
FieldImmunology and Microbiology
TopicNeutrophil, Myeloperoxidase and Oxidative Mechanisms
Canadian institutionsnot available
FundersCanadian Institutes of Health ResearchHospital for Sick ChildrenUniversity of TorontoCystic Fibrosis Canada
KeywordsNeutrophil extracellular trapsTrap (plumbing)ExtracellularChemistryBiologyCell biologyPhysicsImmunologyInflammationMeteorology
DOInot available

Abstract

fetched live from OpenAlex

Immune responses vary between men and women. The female sex is considered protective in combatting acute infections, but disadvantageous in chronic inflammatory diseases. As neutrophils play a central role in innate immunity, they may mediate the sexual dimorphism in immune responses. Neutrophils can combat invading microbes by forming neutrophil extracellular traps (NETs). We hypothesize that females release higher amounts of NETs, leading to more robust acute immune responses. To test our hypothesis, neutrophils isolated from healthy human males and females were induced to form NETs in response to various pharmacological and biological stimuli. Total DNA content was higher in neutrophils from females compared to males. Female neutrophils released decreased levels of DNA in response to the calcium ionophore A23187. PMA, LPS, ionomycin and bacteria stimulation did not result in significant sex differences. Correlating the serum levels of the sex hormones testosterone, estradiol and progesterone to NETosis levels in the above conditions revealed a negative correlation between testosterone and A23187-mediated DNA release. No correlation was detected in relation to endogenous estradiol and progesterone levels, but a combination effect has not been fully explored. Exogenous estradiol or progesterone treatment at physiological concentrations did not influence DNA release. Given the results thus far, a sex difference in NETosis was detected with A23187 treatment but not from biological stimuli in the context of healthy neutrophils.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.009
Threshold uncertainty score0.029

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0090.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.227
Teacher spread0.210 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2018
Admission routes1
Has abstractyes

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