<sup>17</sup>O NMR Studies of Yeast Ubiquitin in Aqueous Solution and in the Solid State
Bibliographic record
Abstract
Abstract We report a general method for amino acid‐type specific 17 O‐labeling of recombinant proteins in Escherichia coli . In particular, we have prepared several [1‐ 13 C, 17 O]‐labeled yeast ubiquitin (Ub) samples including Ub‐[1‐ 13 C, 17 O]Gly, Ub‐[1‐ 13 C, 17 O]Tyr, and Ub‐[1‐ 13 C, 17 O]Phe using the auxotrophic E. coli strain DL39 GlyA λDE3 ( asp C − tyr B − ilv E − gly A − λDE3). We have also produced Ub‐[η‐ 17 O]Tyr, in which the phenolic group of Tyr59 is 17 O‐labeled. We show for the first time that 17 O NMR signals from protein terminal residues and side chains can be readily detected in aqueous solution. We also reported solid‐state 17 O NMR spectra for Ub‐[1‐ 13 C, 17 O]Tyr and Ub‐[1‐ 13 C, 17 O]Phe obtained at an ultrahigh magnetic field, 35.2 T (1.5 GHz for 1 H). This work represents a significant advance in the field of 17 O NMR studies of proteins.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".