Protective effects of Gracilaria lemaneiformis extract against ultraviolet B-induced damage in HaCaT cells
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Background: Gracilaria lemaneiformis is an edible red marine macroalga that contains various active components including phycoerythrin, polysaccharides, and phenolics. In our previous work, crude ethanolic extracts of G. lemaneiformis exhibited potential antioxidative and anti-photoaging activities. Therefore, in this study, we aimed to further investigate the antioxidative and anti-photoaging activities of different fractions of G. lemaneiformis (n-hexane, ethyl acetate, and aqueous fractions [HF, EAF, and AQF, respectively] using ultraviolet B (UVB)-induced HaCaT cells. Materials and Methods: Solvents with different polarity were used to fractionate crude ethanolic extract of G. lemaneiformis. The physicochemical properties of HF, EAF, and AQF were detected by gas chromatography–mass spectrometry, Fourier transform-infrared spectroscopy, and high-performance liquid chromatography–mass spectroscopy. The antioxidant and antiapoptotic effects of the fractions were evaluated by estimating the levels of reactive oxygen species, antioxidant enzymes, and mitochondrial membrane potential (MMP) in UVB-exposed HaCaT cells. Results: According to our results, fatty acids, chlorophyll-a, and soluble polysaccharides were, respectively, present in the HF, EAF, and AQF. Furthermore, both EAF and AQF decreased the UVB-induced apoptosis by decreasing MMP. These results also suggest that EAF and AQF provide cytoprotective effects by activating the antioxidant enzymes. The soluble polysaccharides of AQF and chlorophyll-a of EAF were positively correlated with antioxidant activity. In addition, AQF exhibited stronger antioxidant and anti-photodamage properties than that of other fractions in UVB-radiated HaCaT cells. Conclusion: The results of this study indicated that water-soluble polysaccharides from G. lemaneiformis may be suitable to use as a natural anti-photodamage agent.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it