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Record W3098314736 · doi:10.22215/etd/2020-13985

MGluR5 Modulation Effect on Protein Degradation in Parkinson's Disease

2020· dissertation· en· W3098314736 on OpenAlexaff
Viktoria Xing

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldMedicine
TopicParkinson's Disease Mechanisms and Treatments
Canadian institutionsCarleton University
Fundersnot available
KeywordsDownregulation and upregulationPI3K/AKT/mTOR pathwayAutophagyProtein kinase BCell biologyChemistryNeuroscienceBiologyBiochemistrySignal transduction

Abstract

fetched live from OpenAlex

Parkinson's disease (PD) is the second most common neurodegenerative disease that is characterized by motor symptoms and dysregulation of the dopaminergic system.One of the main hallmarks of PD is abnormal alpha-synuclein (α-syn) aggregation that is the main component of Lewy bodies, the formation of which leads to oxidative stress, excitotoxicity and eventual cell death.In out experiment α-syn fibrils and A53T adenovirus will be added to SH-SY5Y cells to mimic PD conditions of protein aggregation and overexpression.In this thesis investigates the possibility of clearing αsyn fibrils from SH-SY5Y cells using an allosteric mGluR5 inhibitor, CTEP.We found that CTEP contributed to significant elimination of α-syn fibrils form SH-SY5Y cells.Assessment of AKT/mTOR pathway has shown that CTEP action is at least in part mTOR dependent.Additionally, we found a significant upregulation of autophagy system components, such as Beclin1, Atg5-Atg12 complex, Atg7, Atg101 and Atg3, and significant downregulation of inhibitory pULK1 in response to CTEP administration.We conclude that CTEP is a useful pharmacological agent that could be used against the abnormal α-syn aggregation.v Effect of CTEP on Autophagy Elongation ..........

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.021

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0060.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.274
Teacher spread0.261 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2020
Admission routes1
Has abstractyes

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