Characterization of a rice line, ‘Akitasake 44’, with a lower pasting temperature screened from rice lines suited for <i>sake</i> brewing and determination of the responsible gene
Bibliographic record
Abstract
酒米の重要な特性である蒸米の酵素消化性は，澱粉の糊化温度が低い米において優れる．著者らは，代表的な酒造好適米を含む41品種・系統から，澱粉の糊化温度の指標となる粘度上昇開始温度が低い「秋田酒44号」を見出した．この系統のアミロペクチンの側鎖長分布を解析したところ，「あきたこまち」や「五百万石」等と比べ，重合度6から12の短鎖の比率が高く，重合度13から24の中鎖の比率が低かった．また，同系統は胚乳における澱粉代謝系酵素のひとつ，澱粉ホスホリラーゼ1の活性を欠損していた．一方，「秋田酒44号」とその系譜上にある品種・系統について，澱粉ホスホリラーゼ1の活性を調査したところ，活性の欠損は「秋田酒44号」以外には認められなかった．「秋田酒44号」は「58系3071」へのγ線照射によって育成されていることから，澱粉ホスホリラーゼ1活性欠損の原因は，このγ線照射による変異が原因と推定された．さらに，「秋田酒44号」の澱粉ホスホリラーゼ1遺伝子の塩基配列を決定し，同酵素活性を保持する「日本晴」の配列と比較したところ，第12エクソンにアミノ酸置換を伴う一塩基置換があることを確認した．この変異が「秋田酒44号」の澱粉ホスホリラーゼ1活性の欠損，ひいては澱粉糊化時の粘度上昇開始温度が低い原因であると考えられた．
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".