251 Impacts of different levels of dietary zinc oxide on mitochondrial energy metabolism and oxidative stress conditions in post-weaned piglets
Bibliographic record
Abstract
Abstract Zinc oxide (ZnO) is commonly added to weanling piglet diets for its documented benefits as growth promoting agent and usefulness for preventing diarrhea. We have recently reported the occurrence of mitochondrial energetic deficiencies and oxidative stress conditions in post-weaned piglets. In cells, Zn is known to be primarily located in mitochondria, where it is involved in mitochondrial function. The objective of this study was to determine the impacts of different levels of dietary ZnO on mitochondrial function and oxidative stress. One hundred twenty suckling piglets (5.65 ± 0.68 kg) were selected at d14. After weaning (d21), they were fed a basal diet supplemented with 100 mg/kg (low; L), 1000 mg/kg (medium; M) or 3000 mg/kg (high; H) of ZnO until d42 (n = 40/treatment). Piglets were sacrificed at d21, d23, d35, and d42 of age (n = 10/treatment/d) for sample collection. Mitochondrial respiration was evaluated using an extracellular flux analyzer and our results showed higher oxygen consumption rates in liver mitochondria from H group (P < 0.05) at d42. The cellular energy status was characterized by measuring liver adenosine 5’-triphosphate (ATP) content, and lower levels were reported in H group (P < 0.01) at d42. These observations were concomitant with a decrease in activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in cytosol and mitochondria. Quantitative RT-PCR analyses revealed that expression of genes implicated in oxidative stress response such as glutathione synthase (GSS), heme oxygenase 1 (HO1) and metallothionein 3 (MT3) is increased in H group at d42 (P < 0.05). In plasma, GPx activity and oxidative damage to proteins (carbonyls) was higher in H group (P < 0.05). These findings indicate that ZnO affects mitochondrial function and oxidative stress in post-weaned piglets and provide insights to guide the prophylactic practices using high levels of dietary ZnO.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".