Process design of a continuous biotransformation with in situ product removal by cloud point extraction
Bibliographic record
Abstract
Abstract In this work, a continuous, heterogeneous extractive biocatalysis was realized with penicillin G hydrolysis as a model reaction. Therefore, commercially available structured packing CY from Sulzer were coated with enzyme‐containing gels and their catalytic activity was examined. Thereby, the potential of aqueous micellar two‐phase systems (ATPMS) as an extraction medium was evaluated. Triton X‐114 was used as model surfactant. It was found that the separation efficiency of the packing is not affected by the coating and ATPMS allows for a good separation yield. With these results, different process concepts for the continuous, heterogeneous extractive biocatalysis were compared. Overall, a countercurrent process with side inlet ( = 78.1% ± 1.3%) has shown a great potential for a selective product separation and thus, higher yields in comparison to a monophasic batch operation ( = 51.9% ± 0.6%) as well as a biphasic batch operation ( = 67.6% ± 1.0%, wTriton X‐114 = 5%) were achieved.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".