Investigation of stalk formation, frequency of dividing cells and gene expression in periphyton mats dominated by <i>Didymosphenia geminata</i>
Bibliographic record
Abstract
The diatom Didymosphenia geminata can produce thick benthic mats along river bottoms, possibly in association with soluble reactive phosphorus mat-forming genetic variants, or gene expression associated with environmental variation. We isolated cells collected from mat-forming and non-mat-forming sites in British Columbia and Alberta, Canada and compared soluble reactive phosphorus, frequency of dividing cells and gene expression via RNA sequencing. Frequency of dividing cells and soluble reactive phosphorus were higher at the non-mat-forming site and there was no significant difference in frequency of dividing cells between Alberta and British Columbia sites, with one exception. Sixty-six gene products from reference diatom (Phaeodactylum tricornutum and Thalassiosira pseudonana) and bacterial (Caulobacter crescentus) genomes were expressed only in the mat-forming sample and many were related to phosphorus metabolism. One hundred seventy two genes were expressed only in the non-mat-forming sample and many were related to cell division and silica metabolism. Differential gene expression in the periphyton communities suggests that there might be different periphyton molecular phenotypes, including D. geminata, related to P acquisition and polysaccharide metabolism at the low P site and cell division and growth at the high P site.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".