Characterization of the exosomes in the allantoic fluid of the chicken embryo
Bibliographic record
Abstract
The embryo stage is critical for chicken development. Numerous studies have been conducted to clarify the dynamic changes and functions of various proteins and the composition of amino acids during embryo development. However, the physiological characteristics of extraembryonic fluid (allantoic and amniotic), especially allantoic fluid (AF), remain largely unexplored; furthermore, how information is transmitted from embryonic fluid is unknown. In this study, AF-derived exosomes ranging from 60 to 160 nm in diameter from fertilized eggs at 13 d of incubation of fast-growth chickens (WG chicken), medium-growth chickens (Silky N4 chicken), and slow-growth chickens (Huiyang Beard chicken) were isolated and purified by different ultra-centrifugations and further verified by transmission electron microscopy and a flow nano-analyzer. Expression of the exosomal positive biomarkers of ALIX and HSP70 as well as lack of the epithelium marker GRP78 was observed by Western blotting. In addition, small RNA sequencing revealed that AF-derived exosomes at 13 d of incubation contained a large number of known miRNAs (32.62%–65.83%). The top 10 most abundant and co-expressed miRNAs were primarily related to development, growth, and immunity. In addition, AF-derived exosomes promoted DF-1 cell migration. These findings broadened our understanding of the characteristic of AF-derived exosomes.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".