Modification of the Stripe Rust Resistance Gene Yr10 in Triticum aestivum
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Stripe rust is a disease in Triticum aestivum (bread wheat) that is caused by the fungal pathogen Puccinia striiformis. The pathogen has evolved to defeat an R gene in T. aestivum known as the Yr10 gene. The Yr10 gene was found to encode an evolutionary-conserved sequence known as the CC-NBS-LRR. This conserved sequence was found to be involved in producing resistance to various pathogens. Within this sequence the coiled coil (CC) and leucine rich repeat (LRR) domains are thought to be important to the protein’s function. This project made use of PCR overlap-extension mutagenesis to mutagenize the CC and LRR domains in an attempt to create modified constructs of the Yr10 gene. PCR reactions gave fragments of the expected sizes which were then assembled into the pANIC6D vector to be used in transformation. Transformation of the vector with inserted sequences into Escherichia coli will be done to confirm the successful insertion of the fragments. There is hope that in the future the modified constructs could be transformed into wheat. *Indicates presenter
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it