Effects of Crowding and Confinement on Enzyme Kinetics
Bibliographic record
Abstract
Reverse micelles are formed by the aggregation of water inside of a bilayer formed by a surfactant in a non-polar solvent. The nanometer scale pool of water within the reverse micelles provides an environment to study the effects of confinement on enzymes as measured by changes in kinetics. The size of these reverse micelles is directly related to the ratio of water and surfactant. We and others have been studying the effects of crowding, the presence of large concentrations of solutes, on enzyme kinetics. What effect would it have on enzyme kinetics if these solutes were added to the confined environment of the reverse micelle? We have studied the effects of confinement on alpha-chymotrypsin in a system using dioctyl sulfosuccinate (AOT) as a surfactant, isooctane as the non-polar solvent, and N-succinyl-L-phenylalanine p-nitroanilide (SPN) as the substrate. Results will be analyzed within the Michaelis-Menten framework. * Indicates faculty mentor.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".