Study of the Enzyme Activity Change due to Inkjet Printing for Biosensor Fabrication
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Enzymes, the most commonly used biosensing element, have a great influence on the performance of biosensors. Recently, drop-on-demand (DOD) printing technique has been widely employed for the fabrication of biosensors due to its merits of noncontact, less waste, and rapid deposition. However, enzyme printing studies were rarely conducted on the effect of printing parameters from the aspect of the pressure wave propagation mechanism. This study investigated the effects of pressure wave propagation on enzyme activity from the aspects of wave superposition, wave amplitude, resulting mechanical stress, and protein conformation change using pyruvate oxidase as the model enzyme. We found that the mechanical stress increased the activity of pyruvate oxidase during the inkjet printing process. A shear rate of 3 × 105 s–1 enhanced the activity by 14.10%. The enhancement mechanism was investigated, and the mechanical activation or mild proteolysis was found to change the conformation of pyruvate oxidase and improve its activity. This study is fundamental to understand the effect of both printing mechanism and induced mechanical stress on the properties of biomolecules and plays an important role in modulating the activity of other enzyme-based inks, which is crucial for the development of biosensors.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it