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Record W3127395674 · doi:10.1101/2021.01.30.428930

Small Intestinal Resident Eosinophils Maintain Gut Homeostasis Following Microbial Colonisation

2021· preprint· en· W3127395674 on OpenAlexafffund
Kathleen Shah, Aline Ignácio, Jeremiah Bernier‐Latmani, Yasmin Köller, Gillian Coakley, Mati Moyat, Richard C. Hamelin, F. Armand, Nathan C. Wong, Hena R. Ramay, Carolyn A. Thomson, Regula Burkhard, Antoine Dufour, Tatiana V. Petrova, Nicola Harris, Karen D. McCoy

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2021
Typepreprint
Languageen
FieldMedicine
TopicEosinophilic Esophagitis
Canadian institutionsAlberta Bone and Joint Health InstituteUniversity of Calgary
FundersMedical Research CouncilNational Health and Medical Research CouncilNatural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchCumming School of Medicine, University of CalgaryUniversity of CalgarySchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungFundação de Amparo à Pesquisa do Estado de São PauloCanterbury Medical Research FoundationNational Science Foundation
KeywordsEosinophilHomeostasisBiologyCell biologySmall intestineImmune systemImmunologyMotilityIntestinal mucosaExtracellularInternal medicineEndocrinologyMedicine

Abstract

fetched live from OpenAlex

SUMMARY Intestinal homeostasis following postnatal microbial colonization requires the coordination of multiple processes including the activation of immune cells, cell-cell communication, the controlled deposition of extracellular matrix, and epithelial cell turnover and differentiation. The intestine harbors the largest frequency of resident eosinophils of all homeostatic organs, yet the functional significance of eosinophil recruitment to this organ has long remained enigmatic. Eosinophils are equipped to both respond to, and modify, their local tissue environment and thus are able to regulate the adaption of tissues to environmental changes. We report a critical role for eosinophils in regulating villous structure, barrier integrity and motility in the small intestine. Notably, the microbiota was identified as a key driver of small intestinal eosinophil activation and function. Collectively our findings demonstrate a critical role for eosinophils in facilitating mutualistic interactions between host and microbiota and provide a rationale for the functional significance of their early life recruitment in the small intestine. HIGHLIGHTS The microbiota is a critical regulator of eosinophil activation and turnover Eosinophils uphold intestinal barrier integrity following microbial colonization Loss of eosinophils at steady-state results in increased villous blunting and altered intestinal motility

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.012

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.242
Teacher spread0.218 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2021
Admission routes2
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicEosinophilic EsophagitisFrench-language works237,207