Cloning, Expression and Vector Construction of <i>BrSOT16</i> Related to Glucosinolate Synthesis in <i>Brassica rapa</i> ssp. <i>chinensis</i> L.
Bibliographic record
Abstract
Sulfotransferase is an enzyme that catalyzes the transfer of sulfate groups from 3’-phosphoadenosine 5’-phosphosulfate to various receptor molecules, and plays an important role in plant growth and development as well as resistance to diseases and insect pests, etc. In this study, the 1 020 bp gene sequence of BrSOT16 was obtained by homologous cloning from Brassica rapa . Bioinformatics analysis showed that BrSOT16 encoded a stable hydrophilic protein containing 339 amino acids, whose molecular mass and theoretical isoelectric point were 39.25 kD and 5.50, respectively. It was a member of the plant sulfotransferase family containing four conserved regions and having no signal peptide and transmembrane domain. Evolutionary analysis showed that BrSOT16 had the closest relationship with BcSOT16 and BnSOT16 . QRT-PCR analysis showed that BrSOT16 showed an expression pattern that increased first and then decreased as the plants continued to develop, and it had the highest expression level in the leaves of pakchoi at the twelve-leaf stage. The overexpression vector of BrSOT16 was constructed by the method of homologous substitution for the biological function identification, which could provide experimental materials and technical assistance for further study on the genetic engineering of pakchoi.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.004 | 0.006 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".